Thesis (M.Sc.) -- University of Toronto, 1999.
|Series||Canadian theses = -- Thèses canadiennes|
|The Physical Object|
|Pagination||1 microfiche : negative. --|
Hepoxilin A3 blocks the release of norepinephrine from rat hippocampal slices. Biochem Biophys Res Commun. Dec 31; (3)– Carlen PL, Gurevich N, Wu PH, Su WG, Corey EJ, Pace-Asciak CR. Actions of arachidonic acid and hepoxilin A3 on mammalian hippocampal CA1 neurons. Brain Res. Sep 11; (1)–Cited by: History. HxA3 and HxB3 were first identified, named, shown to have biological activity in stimulating insulin secretion in cultured rat pancreatic islets of Langerhans in Canada in by CR Pace-Asciak and JM Martin. Shortly thereafter, Pace-Asciak identified, named, and showed to have insulin secretagogue activity HxA4 and HxB Nomenclature. HxA3, HxB3, and their isomers are CAS Number: Hepoxilin epoxide hydrolase activity was demonstrated in rat liver cytosol using as substrate [C] hepoxilin A3, a recently described hydroxy epoxide derivative of arachidonic acid. The enzyme was isolated and purified to apparent homogeneity using conventional chromatographic procedures resulting in fold purification. Formation of hepoxilins has been demonstrated in platelets,,, lung,, pancreatic islets,, brain, aorta, neutrophils, Aplysia brain, and rat insulinoma lin-like compounds (14,hepoxilins) derived from 15S-HpETE and from docosahexaenoic acid (bis-α-dihomo-hepoxilins) have also been isolated from garlic roots, and pineal glands and brain, respectively.
The uptake into brain was for harmaline slower than for harmine. The rate of elimination was in general smaller for harmaline than for harmine. 3. Harmaline was excreted into chymus and urine to a greater extent than harmine. 4. The binding to proteins of rat plasma was % for . in rat brain synaptosomes and in broken membrane fractions derived from rat brain with a KD of approxi- mately 2 11~ at 36°C. Batrachotoxin and scorpion toxin, which act at different receptor sites to activate sodium channels, have no effect on saxitoxin binding. Osmotic. Hepoxilins are epoxy alcohols synthesized through the lipoxygenase (LO) pathway in animal cells. The epidermis is the principal source of hepoxilins in humans. Here we report on the formation of novel hepoxilin regioisomers formed by the LO pathway in human cells. The Hodgkin lymphoma cell line L possesses high lipoxygenase-1 (LO-1) activity and incubation of . Formation, metabolism and action of hepoxilin A3 in the pineal gland. The term “hepoxilin” was coined in to combine key structural features of the molecule “hydroxy epoxide” with the first demonstrated biological activity “insulin secretion”, hence “H-EPOX-ILIN”,.Two hepoxilins have been isolated and named according to the location of the hydroxyl group at C-8 or C.
P. Shankaranarayanan, R. Ciccoli, S. NigamBiosynthesis of hepoxilins: evidence for the presence of a hepoxilin synthase activity in rat insulinoma cells FEBS Lett., . Table 1. 8S/R‐Hydroxy‐11,12‐epoxyeicosa‐5Z,9E,14Z‐trienoic acid (hepoxilin A 3) (HXA 3) synthase and 12S/15S‐lipoxygenase (LOX) activities of rat 12S‐LOX (wild‐type) and its mutants. Rat 12S‐LOX from pancreatic RINm5F cells and its mutant isoforms were expressed in Escherichia coli and the supernatants obtained following cell lysis were used for determination of the enzyme. The CB 1 cannabinoid receptor in brain is a G-protein-coupled receptor that exists as a protein possessing seven transmembrane helices that span the membrane. The intracellular surface is able to interact with G-proteins of the G i/o family to regulate effector proteins, including adenylate cyclase, Ca 2+ channels, and K + channels, and to stimulate the mitogen-activated protein kinase . Post your questions to our community of million students and teachers. Get expert, verified answers. Learn faster and improve your grades.